RESEARCH INTEREST

Functional genomics of seed composition


RESEARCH

Investigations on reproductive fitness will be conducted using soybean lines that have been modified for improved seed quality characteristics by traditional and molecular techniques. The pathways for optimal soybean development will be characterized and modifications will be studied to monitor the effects of variable seed composition on subsequent reproductive fitness. Developmental responses to stress factors will be characterized and modifications will be determined which suppress (eliminate) the negative correlation between stress and reproductive fitness.


SELECTED PUBLICATIONS

Bilyeu, K., Gillman, J. & LeRoy, A. (2010). Novel FAD3 mutant allele combinations produce soybeans containing 1% linolenic acid in the seed oil. Crop Science. In press.

Lenis, J., Gillman, J., Lee, J., Shannon, J. & Bilyeu, K. (2010). Soybean seed lipoxygenase genes: molecular characterization and development of molecular marker assays. Theoretical and Applied Genetics. 120:1139-1149.

Dierking E, and Bilyeu K (2009). New sources of soybean seed meal and oil composition traits identified through TILLING. BMC Plant Biology, 9(1):89.

Gillman JD, Pantalone VR, Bilyeu K.  (2009). The low phytic acid phenotype in soybean line CX1834 is due to mutations in two homologs of the maize low phytic acid gene. The Plant Genome, 2(2):179-190.

Bilyeu, K.D., Ren, C., Nguyen, N.T., Herman, E. and Sleper, D.A.  (2009).  Association of a four basepair insertion in the P34 gene with the low allergen trait in soybean.  The Plant Genome, 2(2):141-148.

Dierking, E.C. and Bilyeu, K.D. (2009).  Raffinose and stachyose metabolism are not required for efficient soybean seed germination.  Journal of Plant Physiology, 166(12):1329-1335.

Šmehilová, M., Galuszka, P., Bilyeu, K.D., Jaworek, P., Kowalska, M., Šebela, M., Sedlářová, M., English J.T., and Frébort, I.  (2009) Subcellular localization and biochemical comparison of cytosolic and secreted cytokinin dehydrogenase enzymes from maize.  Journal of Experimental Botany. 60:2701-2712.

Ren, C., Bilyeu, K.D., and Beuselinck, P.R. (2009)  Composition, vigor, and proteome of mature soybean seeds developed under high temperature. Crop Sci. 49:1010-1022.

Dierking, E.C. and Bilyeu, K.D. (2008).  Association of a soybean raffinose synthase gene with low raffinose and stachyose seed phenotype.  The Plant Genome 1:136-145.

Flores T, Karpova O, Su X, Zeng P, Bilyeu K, Sleper DA, Nguyen HT, Zhang ZJ (2008). Silencing of Gm FAD3 gene by siRNA leads to low α-linolenic acids (18:3) of fad3 -mutant phenotype in soybean [ Glycine max (Merr.)] Transgenic Research 17:839-850.

Cooper J, Till B, Laport R, Darlow M, Kleffner J, Jamai A, El-Mellouki T, Liu S, Ritchie R, Nielsen N, Bilyeu K, Meksem K, Comai L, Henikoff S (2008). TILLING to detect induced mutations in soybean. BMC Plant Biology 8, 9.

Bilyeu, K.D., Zeng, P., Coello, P., Zhang, Z.J., Krishnan, H.B., Bailey, A., Beuselinck, P.R., and Polacco, J.C. (2008).  Quantitative conversion of phytate to inorganic phosphorus in soybean seeds expressing a bacterial phytase.  Plant Physiology 146, 468-477.